In a tape backup system, this is the point at which you'd insert a new tape - but with D2D (disk to disk) you have to make some changes to the backup concepts.
Here are couple of the points you need to consider...
With tape, the traditional methods use multiple tape cartridges, which are rotated - for example the simplest - grandfather/father/son - three tapes are used in a sequence which allows one to be stored offsite, one on site for immediate restore and one on site for a backup. This allows you to have multiple versions of the backup so that if one becomes contaminated (a virus could be present and be backed up) there are others that can be restored, and if a tape becomes damaged, again there are others that can be restored.
Translating this to disk, you either need removable disk cartridges (they do exist - Imation Odyssey is an example) or you need to create three backup targets on your "fixed' disk and rotate the backups amongst them, overwriting the old backup, the same as would be done with tape)
The "translation" above assumes a full backup, as this is the simplest, but can be used with all forms of backup - full/incremental/differential, and all rotation types - it just becomes a matter of creating and selecting the targets - which can get quite complicated for the basic backup utilities, but can successfully be automated with the more complex (and expensive) ones such as BackUpExec - this is one case where you get what you pay for.
Now - an area that many people overlook when moving to D2D backup - and is vitally important
When you use a single tape drive - your backups are stored on different physical media, a drive failure can be dealt with by replacing the drive, you have the data on the removable media.
If you use a single fixed disk to hold all your targets - the same does not hold true - your backups are on the same physical media, and a drive failure takes your data with it - you need removable media or you need redundancy.
What to do?
Reformat the disks (consider RAID1 if you're not already using it) and reconfigure your backup utility so that it allows you to (a) select multiple target location and (b) overwrites the selected target location.
Can Vista backup do this - I don't know, but if it can't, you'll need to find a backup utility that does.