Yes, I currently have a single 300MB Seagate drive. My network access settings are very simple right now (tired of re-entering a complex setup at each reboot):
  Users: shareuser1, ftpuser1
  Groups: private
  Share:share Volume_1/share 
  User/Group: shareuser1
  Comment: Network Share
  Oplocks: no
  Map: no
  R/W: Read Only
Yes, it happens on every single reboot.
Yes I do have a case # for the phone call. Why?
Was the DNS-321 never tested with a single drive even though it is documented as a supported configuration on the user manual?
-Dan