The Problem you are having is simply the Media server software you are using does not support .mkv. If the server software does not support .mkv the DSM-750 cannot see those file available to play.
ECF...your assertion is, quite simply, incorrect. Both Tversity and PS3 software server applications expressly support .mkv and, in fact, begin to stream it wirelessly to the DSM 750 after first transcoding the files. Unfortunately, this is, however, where the problem apparently occurs in that the concurrent transcoding and streaming is not 100% foolproof and eventually (anywhere from 3 to 10 minutes into the video) will begin to stutter or, worse still, freeze up entirely. Of course, this might depend on the speed of your individual processor, which might explain why the guys who are using a separate dlink DNS server to store these files first are not having this problem But I did not plan on spending even more money to buy a separate server for this purpose, especially when dlink advertises the ability of the DSM 750 to stream .mkv files wirelessly from one's computer and doesn't even mention the requirement of such additional hardware.
Just to set the record straight for everyone on this forum (especially ECF as a dlink representative/moderator), I spoke to a dlink Level 3 Tech today named Alex in the company's Huntingdon Valley headquarters about this issue. In turn, he consulted their project management team of experts for the DSM 750. They all acknowledged (or conceded...if you will) that the dlink media server software does not now and may not in the future support .mkv files. That is why they refer to other server applications in their owner manual as possible alternatives. Moreover, they agreed that these other existing alternate applications may not work correctly on all computer systems, as I and many others have already recognized. At present, they stand by their advertising statement that the DSM 750 "supports" .mkv or .h264 files in that these files can be played perfectly by use of the USB port feature.
I'll leave it to everyone to form their own opinion on whether this type of qualification is expressly explained in their advertising promotions or whether dlink is being deliberately deceptive to appear to have the only wireless media product capable of streaming .mkv files. As for me, I have concluded that its just as easy to plug my laptop into my HDTV with an HDMI jack as it is to copy the .mkv video file onto a flashdrive and insert it into the DSM 750. Further, I really miss the "jump" feature and volume adjustment on my old DSM 520, the elimination of which from the DSM 750 really makes no sense to me.
That said, I have decided to return the DSM 750 and wait for software server technology to catch up to dlink's hardware, so the product will actually work as advertised right out of the box. Hopefully, with the speed of technology today, I won't have to wait too long. Meanwhile, I just wish dlink would, at least, reimburse me for the 15% restocking fee I will have to pay when I return the unit for a refund.
As the say in law school, caveat emptor or let the buyer beware!!!!!!!!!!!
Parenthetically, it seems worth mentioning that Divx has actually released a server application capable of streaming .mkv files to a "Divx Connected Device." Their web site actually links to the dlink DSM330 as just such a device, so at some point in the past Divx and Dlink worked together to try to bridge this gap. Unfortunately, that partnership appears to have been abandoned now and Dlink no longer supports Divx (as opposed to Xvid), which is a shame because Divx may be one of the first companies to produce a codec capable of resolving this transmission/streaming issue.